Fig 2: Associations between the DEGs and patient outcomes in liver cancer. Overall survival curve for patients with liver cancer in the GEPIA database (right) and overall survival and disease specific survival curves (left, center) for patients with liver cancer in the Kaplan-Meier plotter databases stratified by expression of (A) POSTN, (B) LAYN, (C) HTRA3, (D) AANAT, (E) AFM, and (F) NAPB.

Fig 3: TMAO and TNF-a alter expression of the DEGs in Hepa1-6 cells and Huh7 cells. (A–D) Expression of the POSTN, NAPB, LAYN and HTRA3 mRNAs were significantly upregulated in Hepa1-6 cells and Huh7 cells treated with 50 ng/ml TNF-a and 50 µM TMAO in combination compared to cells treated with 50 ng/ml TNF-a alone; results are mean ± SD (n = 3 per group); Student’s t-test. (E,F) On the contrary, 50 ng/ml TNF-a and 50 µM TMAO in combination significantly downregulated the expression of AANAT and AFM compared to cells treated with 50 ng/ml TNF-a alone; results are mean ± SD (n = 3 per group); Student’s t-test.